What is the purpose of incubating the unopened plates?
What is the purpose of incubating the unopened plates?
The purpose of incubating unopened plates is to check in there is contamination in the media preparation phase.
How does the growth on the unopened plates affect the reliability of the other plate?
How does growth on the unopened plates affect the reliability (your interpretation) of the other plates? Contamination when agar was plated could cause contamination in all or just one plate. Growth on unopened plates means the other plates could also be contaminated and the results are not sound.
What is the likely source reservoir of microbes that grow best at 37oc?
So the source of microorganisms at 35 degree Celsius is human body. They survive because microbes are inert and nutrients present which are not seen to our eyes. Bacteria have spores as a stage of their life cycle. Spores can survive without water or nutrients for long time that may be some of them for centuries.
What is the ubiquity of microorganisms?
Microorganisms are ubiquitous; that is, they are present nearly everywhere. In this lab you will try to isolate bacteria and other microorganisms from various sources using different types of media.
Were the three different streak methods?
There are many different types of methods used to streak a plate. There are two most commonly used streak patterns, a three sector “T streak” and four-quadrant streak methods. Picking a technique is a matter of individual preference and can also depend on how large the number of microbes the sample contains.
What is the reason for the zigzag streaking pattern?
As you might guess, the purpose of streaking for isolation is to produce isolated colonies of an organism on an agar plate. This is useful when you need to separate organisms in a mixed culture or when you need to study the colony morphology of an organism.
What are the different goals between a streak plate and a pour plate?
The main difference between streak plate and pour plate is that in streak plate, the first to be added is the melted nutrient agar and the second to be added is a loop of bacteria from a slant, whereas the first to be added in pour plate is the bacterial broth and the second to be added is the nutrient agar.
What is meant by a bacterial lawn?
Bacterial lawn is a term used by microbiologists to describe the appearance of bacterial colonies when all the individual colonies on a Petri dish agar plate merge to form a field or mat of bacteria.
What does lawn mean?
(Entry 1 of 2) 1 archaic : an open space between woods : glade. 2 : ground (as around a house or in a garden or park) that is covered with grass and is kept mowed. 3 : a relatively even layer of bacteria covering the surface of a culture medium.
What does semi confluent mean?
not comparable