What special procedures should be taken when using the immersion oil lens?

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What special procedures should be taken when using the immersion oil lens?

Using immersion oil Place a drop of immersion oil on the cover slip over that area, and very carefully swing the oil immersion lens into place. Focus carefully, preferably by observing the lens itself while bringing it as close to the cover slip as possible, then focusing by moving the lens away from the specimen.

What is the oil immersion procedure and why would you use it?

In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.

What should you do before the oil immersion lens is rotated into place?

Lab Test 1 part 1

Question Answer
The most useful adjustment for increasing image contrast in low power magnification is: Answer: closing down the diaphragm
Before the oil immersion lens is rotated into place, you should: Answer: center the object of interest in the preceding lens and place a drop of oil on the slide

What is the proper way to focus a microscope?

  1. Start by rotating the objective lens to lowest power.
  2. Place a slide on the stage, label side up, with the coverslip centered.
  3. On LOW POWER ONLY, use the coarse focus knob to get the object into focus.
  4. If you cannot see anything, move the slide slightly while viewing and focusing.

What are the precautions when using a microscope?

Safety Tips With Microscopes

  • Wear Protective Clothing. Before you begin using a microscope, put on protective clothing.
  • Carry with Two Hands.
  • Do Not Touch the Lens.
  • Do Not Look into the Light.
  • Be Cautious Handling Slides.
  • Storing.

What are 2 safety rules for using a microscope?

Microscope Safety

  1. Clean the microscope after each use.
  2. Handle glass slides carefully.
  3. Turn off the light source when the microscope is not in use.
  4. Be aware if your microscope has a mercury lamp.
  5. When carrying the microscope, always use two hands with one hand supporting the base and theother hand holding the arm.

What is the first thing to do when using a microscope?

How To Use Your First Microscope

  1. Step 1: Moving Your Microscope. Carry the microscope with two hands.
  2. Step 2: Microscope Lens Care. Never touch any lens with your fingers.
  3. Step 3: Microscope Parts.
  4. Step 4: Prepare a Slide.
  5. Step 5: Insert the Slide.
  6. Step 6: Set Up for Viewing.
  7. Step 7: Light Control.
  8. Step 8: Focus the Microscope.

When switching to a 100x lens What should you use?

When switching to the 100x lens, what should you use? 100x lenses should be used with a few drops of immersion oil to enhance the image.