Which types of media are inoculated with an inoculating loop?
Which types of media are inoculated with an inoculating loop?
Types of Media Used for Inoculation
- Agar Plates.
- Broth Culture.
- Slant culture.
- Plate culture.
- Stab culture.
Why do we inoculate the slant surface in a zigzag motion?
Making a Slant to Slant Transfer The zig-zag pattern allows for abundant growth for the creation of many future subcultures while the straight line slant is best for maintaing a stock culture. The source culture for a new slant can be an isolated colony from a streak plate, from another slant or from a broth culture.
For what type of specimen in terms of cell numbers would the zig zag method be most appropriate?
The zigzag streak is used for samples with expected low cell densities, so its appropriateness will depend entirely on the source each student sampled. Most colonies on streak plates grow from isolated colony-forming units (CFUs).
What are the oxygen requirements of bacteria?
Bacteria that require oxygen to grow are called obligate aerobic bacteria. In most cases, these bacteria require oxygen to grow because their methods of energy production and respiration depend on the transfer of electrons to oxygen, which is the final electron acceptor in the electron transport reaction.
What is the best way to determine oxygen requirements?
An excellent way to determine the oxygen needs of your bacterium is to grow it in different oxygen environments—atmospheric oxygen of 22%, no oxygen at all (GasPak jar), and reduced oxygen at less than 10% (candle jar)–and compare the quality and quantity of growth.
What type of media would you use to determine the oxygen requirements of bacteria?
Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes.
What temperature does bacteria like best?
Each type of bacteria grows best in a certain temperature range. Not all bacteria have the same temperature requirements, but most grow best between 40°F and 140°F, which is known as the TEMPERATURE DANGER ZONE.
What are the nutritional requirements for bacterial growth?
For growth and nutrition of bacteria, the minimum nutritional requirements are water, a source of carbon, a source of nitrogen and some inorganic salts. Water is the vehicle of entry of all nutrients into the cell and for the elimination of waste products.
What are the three nutritional types of bacteria?
| Nutritional Type | Energy Source | Examples |
|---|---|---|
| Photoheterotrophs | Light | Some Purple and Green Bacteria |
| Chemoautotrophs or Lithotrophs (Lithoautotrophs) | Inorganic compounds, e.g. H2, NH3, NO2, H2S | A few Bacteria and many Archaea |
| Chemoheterotrophs or Heterotrophs | Organic compounds | Most Bacteria, some Archaea |
What are the 4 sources of nutrition for bacteria?
Bacteria require sources of carbon, nitrogen, phosphorous, iron and a large number of other molecules. Carbon, nitrogen, and water are used in the highest quantities. The nutritional requirements for bacteria can be grouped according to the carbon source and the energy source.
What are some examples of nutritional factors impacting bacterial growth?
Some of the important factors affecting bacterial growth are:
- Nutrition concentration.
- Temperature.
- Gaseous concentration.
- pH.
- Ions and salt concentration.
- Available water.
What are the 4 major domains of microbes?
They include bacteria, fungi, algae, and protozoa; microscopic plants, and animals. Single-celled microorganisms were the first forms of life to develop on earth, approximately 3 billion–4 billion years ago.
What are the main sources of microbial nutrition?
The sources of common essential nutrients are carbon, hydrogen, nitrogen, oxygen, phosphorus, and sulfur. Organisms usually absorb carbon when it is in its organic form. Carbon in its organic form is usually a product of living things.
What are 3 factors that influence the growth and survival of microbes?
Warmth, moisture, pH levels and oxygen levels are the four big physical and chemical factors affecting microbial growth. In most buildings, warmth and moisture are the biggest overall issues present.
How does temperature influence microbial growth?
Physical Factors that Control Microbial Growth. Generally,an increase in temperature will increase enzyme activity. But if temperatures get too high, enzyme activity will diminish and the protein (the enzyme) will denature. On the other hand, lowering temperature will decrease enzyme activity.
How does oxygen affect microbial distribution?
Microorganisms vary in their requirements for molecular oxygen. Obligate aerobes depend on aerobic respiration and use oxygen as a terminal electron acceptor. They cannot grow without oxygen. Obligate anaerobes cannot grow in the presence of oxygen.