Why is it important to flame the mouth of the tube before and after every inoculation?
Why is it important to flame the mouth of the tube before and after every inoculation?
very briefly flame the lip of the culture tube. This creates a convection current which forces air out of the tube and prevents airborne contaminants from entering the tube. keeping the culture tube at an angle, insert the inoculating loop and remove a loopful of inoculum.
Which term is used for the presence of unwanted microbes in a culture?
Aseptic technqiue maintains procedure with pure cultures to transfer the bacteria in a sterle way to see the growth in a pure culture. Techniques that prevent outside organisms from contaminating transfer culture.
What are the methods of culturing microorganisms?
There are several types of bacterial culture methods that are selected based on the agent being cultured and the downstream use.
- Broth cultures.
- Agar plates.
- Agar based dipsticks.
- Stab cultures.
- Culture collections.
- Solid plate culture of thermophilic microorganisms.
- Isolation of pure cultures.
Which instrument is used to grow bacteria?
Instrument list
| Instrument | Uses |
|---|---|
| Hungate Anaerobic tubes | for culturing of anaerobic microbes |
| Incubator | used for bacterial or fungal cultures |
| Inoculation loop: | used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. |
What are the culture media used in microbiology?
List of culture media used in microbiology with their uses
| Media | Uses |
|---|---|
| · Crystal violet blood agar | Selective media for Streptococcus pyogenes |
| · Cysteine Lactose Electrolyte Deficient Media (CLED Media) | Most commonly used media for culturing urine samples |
| · Egg Saline Medium | Preservation of cultures of Gram-negative bacilli |
What is media used for in microbiology?
Microbiological Cultures Culture medium or growth medium is a liquid or gel designed to support the growth of microorganisms. There are different types of media suitable for growing different types of cells. Here, we will discuss microbiological cultures used for growing microbes, such as bacteria or yeast.