Why is it important to store plates in an inverted position when they are being incubated?
Why is it important to store plates in an inverted position when they are being incubated?
Why are inoculated plates incubated in an inverted position? To prevent condensation of water on the lid. Water can mix in with the bacteria not allowing for individual colony growth. -Sometimes water will need to be added to incubators if humidity is required.
Why do we incubate plates upside down quizlet?
Why do we incubate the agar plates upside down? The agar plates were incubated upside down in order to prevent the condensation formed on the lid from dripping thus drowning the colonies.
How will a streak plate look like if the loop had not sterilized between streaking into the new areas of the plate?
What would a streak plate look like if you had not sterilized the loop between streaking into the new areas of the plate? While the number of bacteria colonies seen in each area may decrease, the numbers would not decrease enough for individual colonies to form.
How do you know if the streak plate method was performed correctly?
If you streaked correctly, you will see isolated colonies in the third sector. The heaviest growth will be in the first sector. There will be less growth and some isolated colonies in the second sector. The third area should have the least growth with isolated colonies.
What is the purpose of a 3 or 4 quadrant streak plate?
As the original sample is diluted by streaking it over successive quadrants, the number of organisms decreases. Usually by the third or fourth quadrant only a few organisms are transferred on the inoculating loop and these produce a few isolated colonies.
What is the point of doing a streak for isolation 4 quadrant streak plate?
As you might guess, the purpose of streaking for isolation is to produce isolated colonies of an organism on an agar plate. This is useful when you need to separate organisms in a mixed culture or when you need to study the colony morphology of an organism.
What is the spread plate method used for?
The spread plate method is a technique to plate a liquid sample containing bacteria so that the bacteria are easy to count and isolate. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate.
How much of sample is added in a plate to prepare spread plate technique?
*Note: The volume of the liquid should be 0.1 ml or less….Procedure for Spread Plate Technique
- Prepare a series of at least 6 test tubes containing 9 ml of sterile distilled water.
- Using a sterile pipette, add 1ml of sample in the first tube of the set.
- Mix the contents well by swirling the tube upside down a few times.
Why do you incubate plates at two different temperatures?
The primary reason for incubating bacterial cultures at different temperatures is that specific bacteria are adapted to grow best at different temperatures.
Why was 37 C the optimal incubation temperature?
Why was 37c the optimal incubation temperature? Because it was boiled and raised the temperature.