Why is the streak plate preferred over?

Why is the streak plate preferred over?

why is streak plate preferred over spot inoculation in Blood Agar procedure? Quadrant streak is better for colony isolation as you start out with a much denser same and then isolate it down, making the test more reliable.

Why would you use a streak plate technique to inoculate the throat culture sample instead of using a spot inoculation method?

Why is the streak plate method preferred over the spot inoculation in blood agar? Because the sample from the throat is not a pure culture, so isolation is needed to identify the individual colonies.

Why is it important to streak and stab the bacteria while inoculating the blood agar plate?

Streak-stab technique Often when inoculating a BAP to observe hemoloysis patterns, investigators will also stab several times through the agar using an inoculating loop. This stab allows for the detection of streptolysin O, a specific hemolysin produced by Streptococcus pyogenes.

Why do we need to transport the throat swab using transport culture medium?

Transport medium aims to preserve a specimen and minimize bacterial overgrowth from the time of collection to the processing of the specimen. Depending on the type of organisms suspected in the sample, transport media may vary.

What is the medium of transport?

An agency by which something is accomplished, conveyed, or transferred: The train was the usual medium of transportation in those days.

What are the precautions when handling transporting and processing the specimen?

Specimen collection and processing procedures

  • Avoid patient identification errors.
  • Draw the tubes in the proper sequence.
  • Use proper containers for collection.
  • Mix all tubes ten times by gentle inversion immediately after collection.
  • Do not decant specimens from one type of container into another.

What are the reasons for specimen rejection?

The most common reasons for specimen rejection were contamination (n=764, 35.1%), inappropriate collection container/tube (n=330, 15.2%), quantity not sufficient (QNS) (n=329, 15.1%), labeling errors (n=321, 14.7%), hemolyzed specimen (n=205, 9.4%), and clotted specimen (n=203, 9.3%).

What does specimen handling mean?

More information in Books or on. Definition: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.

When should a specimen be Analysed?

In general, specimens for determining the concentration of body constituents should be collected when the patient is in a basal state (ie, in the early morning after awakening and about 12 to 14 hours after the last ingestion of food).

What are the types of specimen collection?

The types of biological samples accepted in most clinical laboratories are: serum samples, virology swab samples, biopsy and necropsy tissue, cerebrospinal fluid, whole blood for PCR, and urine samples. These are collected in specific containers for successful processing in the laboratory.

What are the characteristics of a specimen?

What characteristics should a specimen have to be suitable for microscopy?

  • Thin: the specimen must be sufficiently thin to be placed on a slide with a cover glass on top.
  • Translucent: The specimen must allow light to go through (it must be translucent).
  • Contrast: The specimens must show sufficient color contrast.

What is the difference between sample and specimen?

A sample is a subset taken for some purpose from a larger population. A specimen is an individual member of the sample. For example, we might take a sample of 3 rocks from a bag.

What are the blood containers called?

Plasma Preparation Using a Plasma Preparation Tube (PPT™) The BD Vacutainer® Plasma Preparation Tube (PPT™) is a plastic evacuated tube used for the collection of venous blood in order to prepare undiluted plasma for use in molecular diagnostic testing.

How long is blood good for in a tube?

Tubes of blood are to be kept closed at all times. 2. Whole blood samples should not remain at room temperature longer than 8 hours. If assays are not completed within 8 hours, samples should be stored at +2°C to +8°C no longer than 7 days.

What is the purple blood tube for?

Overview. Purple blood bottles are generally used for haematology tests where whole blood is required for analysis.

What does the yellow blood tube test for?


Green Sodium heparin(100 USP Units) Ammonia, Lactate, HLA Typing
Tan K2 EDTA Lead levels
Yellow ACD Solution A consists of trisodium citrate, citric acid and dextrose DNA Studies, HIV Cultures
Pink (K2)EDTA Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load

What color tube is used for STD testing?

Specimen Requirements Collect specimen in lavender-top (EDTA), yellow-top (ACD), red-top tube, or gel-barrier tube.

What color tube is used for testosterone?

ORDERING INFORMATION: Geisinger Epic Procedure Code: LAB3137 Geisinger Epic ID: 34071
Preferred collection container: 6 mL red-top (plain, non-serum separator) tube
Alternate Collection Container: 4 mL green/green-top (sodium heparin) tube 3 mL green/black top lithium heparin tube

What test goes in what color tube?

Blood Collection Tubes

Tube cap color Additive Common laboratory tests
Lavender or pink Potassium EDTA Hematology and blood bank
Gray Sodium fluoride, and sodium or potassium oxalate Glucose (especially when testing will be delayed), blood alcohol, lactic acid

What color tube is used for sedimentation rate?

Whole Blood (EDTA) 5 mL whole blood in a lavender-top (EDTA) tube (1.5 mL min). Gently invert tube 8-10 times immediately after drawing to prevent clotting.

What happens when a green topped vacutainer tube is underfilled?

When the ratio of EDTA to blood is too high, as in an underfilled tube, the red cells tend to shrink. As a result, hematocrit, mean cell volume (MCV), and the mean corpuscular hemoglobin concentration (MCHC) will be affected.

What color tubes are drawn last?

1. Collect citrate tube (blue top) last, after 20mL of blood has been withdrawn for other testing, or as waste. Just prior to drawing the blue top tube as the last tube, draw 1mL into a discard blue top tube to prevent cross contamination from the additive of previously drawn tubes.

What is the correct order of draw tubes only?

This standard recommends that EDTA tubes be drawn first to ensure good quality specimen, followed by other additive tubes and finally, serum specimen tubes.

What is the correct order of blood draw tubes?

The draw order for specimen tubes is as follows: Gold SST (Plain tube w/gel and clot activator additive) Green and Dark Green (Heparin, with and without gel) Lavender (EDTA) Pink – Blood Bank (EDTA)

What color tube is a BMP drawn in?

What color tube is used for a basic metabolic panel? Red-top tube or green-top (lithium heparin) tube is acceptable. Gel-barrier tube (send entire tube) is preferred. Red-top tube or green-top ( heparin) tube is acceptable.