Is af488 same as FITC?

Is af488 same as FITC?

Fluorescent conjugates The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.

Can you use APC and PE together?

In my experience, it is not advisable to use APC/Cy7 and PE/Cy7 together due to heavy cross-beam contamination as their emission max are exactly same. However, they can be used together if you are using machines like the Cytek Aurora.

Does PE and APC overlap?

What is the excitation and emission spectrum of PerCP?

PerCP is a protein complex with a molecular weight of approximately 35 kDa. When excited by light at 488 nm from an argon-ion laser, PerCP has a excitation maximum around 490 nm, with an emission spectrum which peaks at 675 nm. The emitted light is collected in the fluorescence-3 (FL3) channel.

How much does PerCP have in molecular weight?

PerCP is a protein complex with a molecular weight of approximately 35 kDa. When excited by light at 488 nm from an argon-ion laser, PerCP has a excitation maximum around 490 nm, with an emission spectrum which peaks at 675 nm.

Which is brighter PerCP cyanine or PerCP-eFluor 710?

PerCP-eFluor 710 The PerCP-eFluor 710 tandem dye was developed as an optimal choice for the FL3 channel on a blue laser line. It is consistently two to three times brighter than PerCP-Cyanine5.5 when evaluated in clone-to-clone comparison tests on identical samples.

Where can peridinin-chlorophyll-protein ( PerCP ) be found?

PerCP Technical Information. Peridinin-Chlorophyll-Protein (PerCP) is a 35.5 kDa fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates. Jackson ImmunoResearch offers the form found in Dinophyceae sp. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission peak at 677 nm.