What is TGX Stain-Free gel?
What is TGX Stain-Free gel?
What Is Stain-Free Technology? Mini-PROTEAN TGX Stain-Free Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.
How do Stain-free gels work?
Stain-Free imaging technology utilizes a polyacrylamide gel containing a proprietary trihalo compound to make proteins fluorescent directly in the gel with a short photoactivation, allowing the immediate visualization of proteins at any point during electrophoresis and western blotting.
What is a Stain-free gel?
Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a fluorescent product when covalently crosslinked to protein tryptophan residues.
How do you make acrylamide gel?
The gel will quickly polymerize! Pipet the stacking gel on top of the polymerized separation gel. Insert a comb (corresponding to the gap between the glass plates) to create either 10 or 15 wells. Wait till the stacking gel is completely polymerized….Making and running an acrylamide protein gel V. 1.
| A | B | |
|---|---|---|
| 5 | dH2O | 0.9 L |
What are TGX gels?
Long shelf life Mini-PROTEAN® TGX™ Precast Gels are an innovative PAGE system designed to provide very fast separation while maintaining high resolution in standard Tris-glycine buffer. Mini-PROTEAN Precast Gels are compatible with Mini-PROTEAN Tetra (1–4 gels) and Mini-PROTEAN® Dodeca™ (1–12 gels) Cells.
What is a stain-free blot?
Stain-Free technology allows scientists to visualize strong total protein signals with low background on their gels and blots that would otherwise not be possible without additional staining steps thus, improving overall dynamic range without the need to perform extra staining and destaining steps.
What is Coomassie blue staining?
Description. Coomassie blue dyes are a family of dyes commonly used to stain proteins in SDS-PAGE gels. The gels are soaked in dye, and excess stain is then eluted with a solvent (“destaining”). This treatment allows the visualization of proteins as blue bands on a clear background.
What does stain-free mean?
(of a fabric, carpet, surface, etc) designed or having added chemicals in order to be resistant to being stained or marked.
How are polyacrylamide gels made?
Polyacrylamide gels are prepared by free radical polymerization of acrylamide and a comonomer crosslinker such as bis-acrylamide. Polymerization is initiated by ammonium persulfate (APS) with tetramethylethylenediamine (TEMED) as the catalyst (see figure below).
What is a 4/20 gradient gel?
Precast gradient gels are solid, polymerized acrylamide slabs with a gradually increasing concentration. The pore size is large at the top (at 4%) and decreases near the bottom (at 20%), gradient gels can resolve the separation of a broader range of different molecular weights.
What are pre cast gels?
GenScript’s Bis-Tris precast gel series are high performance polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability.